Metagenomic deep sequencing reveals association of microbiome. First report of an oxa48 and ctxm2producing kluyvera spp. The gene sequences encoding ctxm enzymes show a high similarity to those of. Putative horizontal transfer of carbapenem resistance between klebsiella pneumoniae and kluyvera ascorbata during abdominal infection. Kluyvera ascorbata has become increasingly significant due to its potential to cause a wide range of infections, as well as its ability to transfer gene encoding for ctxm type extended spectrum blactamases esbls to other enterobacteriaceae. Apr 15, 2020 author summary the physiological mechanisms by which insects survive at low temperatures have not yet been discovered.
By combining transcriptomic and metabolomic approaches, we found that the presence of gut symbionts, especially k. Sputum 28 2 2 urine 1i 2 stool 8 throat 5 1 2 blood 1 1 other,ornotgiven 8 1. Foxit software is the reliable source for fast, affordable, and secure pdf solutions. List of bacteria and similar organisms presenting at the wild. The kit contains 16 biochemical tests, which are placed in 2 rows doublestrips of a divided microplate. Kluyvera ascorbata sud165 and a siderophoreoverproducing mutant of this bacterium, k. Mergepdf is an ultimate tool to merge two or more pdf files into single pdf document. The aim of this study was to determine the ability of a disc susceptibility test using faropenem 10. This bacteria is usually considered a commensal, however it can cause significant infections rarely. Kluyvera ascorbata kluyvera cryocrescens kluyvera intermedia leclercia adecarboxylata moellerella wisconsensis morganella morganii ssp.
Kluyvera mediastinitis following openheart surgery. In october 2006, epa published a notice in the federal register usepa, 2006 requesting chemical and microbial contaminant nominations as part of the process to identify emerging contaminants that. What links here related changes upload file special pages permanent link page. In this work we have redesigned our previously reported 1 universal ctxm primers based on comprehensive analysis of the sequences of new ctxm variants, including members of the growing ctxm8 cluster ctxm40 and ctxm41 as well as of the novel cluster comprised by the ctxm25 and.
The eazyplex superbug cre system correctly detected bla carbapenemase genes with or without bla ctxm genes in 100% of the molecularly characterized strains. Kluyvera ascorbata kluyvera cryocrescens leclercia adecar boxylata moellerella wisconsensis morganella morganii ssp morgani i morganella morganii ssp sibonii pantoea agglomerans pantoea spp proteus mirabilis proteus vulgaris group proteus penneri providencia alcalifaciens providencia rettgeri providencia rustigianii providencia stuartii. Isecp1b is an insertion element associated with the emerging expanded. Pdf infections due to kluyvera species ascp check sample mb 104. Shewanella oneidensis mr1, kluyvera ascorbata atcc 33433, klebsiella aerogenes kctc 2190, kluyvera. First report of an oxa48 and ctxm2producing kluyvera. A comparative phylogenetic analysis based on these 16s rrna gene sequences and those available for strains belonging to several genera of the family enterobacteriaceae showed that members of the genus kluyvera form a cluster that contains all the known kluyvera species. The majority of isolates for which sampling dates were available 80%, were collected between 2012 and 2016, with the oldest available isolates dating back to 2008 fig. Kluyvera ascorbata atcc 33433 not grown not grown not grown kluyvera cryocrescens atcc 33435 not grown not grown not grown. Kluyvera ascorbata infection in a neonate ochi 2017.
Farmer jj, fanning gr, huntleycarter gp, holmes b, hickman fw, richard c, et al. Plesiomonas shigelloides proteus hauseri proteus mirabilis proteus penneri proteus vulgaris. Molecular characterization of long direct repeat ldr. Clinically significant kluyvera infections a report of seven cases. An elderly male presented with multiple pressure sores after being found down for an unknown time period. Epa is including twelve pathogens on the final candidate contaminant list 3 ccl 3, one more than the eleven pathogens on the draft ccl 3. Nosocomial bacteremia due to kluyvera cryocrescens. Pdf kluyvera ascorbata as a pathogen in adults and. Kluyvera 2 kluyvera ascorbata 2 kluyvera cryocrescens 2 kluyvera georgiana 2 kluyvera intermedia kluyvera ascorbata and kluyvera georgiana, the latter two being the progenitor of bla ctxm genes. Gut microbiota promotes host resistance to lowtemperature.
Case report open access kluyvera ascorbata septicemia in. Foxit phantompdf standard is the industrys premiere pdf editor making editing pdf files easier than ever before to deliver professional looking pdf. Pdf kluyvera mediastinitis following openheart surgery. Kluyvera cryocrescens infection has been considered rare.
The cellular fatty acid methyl ester fame composition of buttiauxella spp. In order to assess the relationship between the genus kluyvera and other members of the family enterobacteriaceae, the 16s rrna genes of type strains of the recognized kluyvera species, kluyvera georgiana, kluyvera cochleae, kluyvera ascorbata and kluyvera. Enterobacter asburiae n 4 ndm n 180 escherichia hermannii 3 180 100 enterobacter aerogenes n 8 2018 but has now subsided. Infections caused by kluyvera species in humans clinical. Multidrug resistant kluyvera ascorbata septicemia in an. Antimicrobial activity of ceftolozanetazobactam tested. Detection of protozoan and bacterial pathogens of public. Kluyvera ascorbata, the type species of the genus and.
Kluyvera as a separate genus, using biochemical and dnadna hybridization techniques, have more strains been recognized and more clinical experiences emerged from reports of infections caused by this organism. Kluyvera species soft tissue infection journal of clinical. In addition, there are no reports to verify the role of gut symbionts in the b. Although it causes infections infrequently, it is responsible for causing a wide range of infections. Klebsiella oxytoca, klebsiella pneumoniae ssp pneumoniae, kluyvera ascorbata, leclercia adecarboxylata, morganella morganii ssp morganii, morganella morganii ssp sibonii, proteus myxofaciens, rahnella aquatilis, salmonella cholerasuis ssp cholerasuis, salmonella paratyphi a 2 eliminated 32. Resistances bacteries version 2019 abiotrophia defectiva abiotrophia spp achromobacter denitrificans achromobacter spp achromobacter xylosoxidans citrobacter amalonaticus. A collection of 166 isolates of carbapenemaseproducing enterobacteriaceae cpe and 82 isolates of enterobacteriaceae that produced other. A comparative phylogenetic analysis based on these 16s rrna gene sequences and those available for strains belonging to several genera of the family enterobacteriaceae showed that members of the genus kluyvera. Kluyvera strains ferment many of the sugars and polyhydroxyl alcohols used in identification. Kluyvera ascorbata sepsis in an extremely low birth weight infant. It is believed to be the source of genes encoding ctxmtype extended spectrum blactamases esbls and it has the ability to transfer these genes to other. Kluyvera 2 kluyvera ascorbata 2 kluyvera cryocrescens 2 kluyvera georgiana 2 kluyvera intermedia pdf infections due to kluyvera species ascp check sample.
Kluyvera is a relatively newly described genus in the family. It is gramnegative, rodshaped and motile with peritrichous flagella. Expec strains that are resistant to antimicrobials have been associated with excess mortality, prolonged hospital. Complete assembly of escherichia coli sequence type 1.
Kluyvera ascorbata belongs to enterobacteriaceae family and is a gram negative microorganism. Putative horizontal transfer of carbapenem resistance between. Evaluation of the eazyplex superbug cre system for rapid. Urosepsis caused by kluyvera ascorbata in a pregnant woman. Kluyvera ascorbata is a bacterium, the type species of its genus.
A plasmidborne shewanella algae gene, qnra3, and its. M positive strains may be identified from humans and animals, the ability of this insertion sequence to mobilize the bla ctx. Kluyvera ascorbata is proposed as the type species for the genus. D probe in the chromosomes of salmonella typhimurium, kluyvera ascorbata, leclercia adecarboxylata and citrobacter freundii.
Improved pcr detection and subtyping of ctxmbetalactamase. Mergepdf combine multiple pdf files into single pdf document. In humans, sputum is the most common specimen yielding kluyvera. Soil is the most diverse environment, and genes found in the human gut are also detected in soil, emphasizing its resistance gene reservoir role for pathogens. May 08, 2019 the incidence of infections caused by extraintestinal escherichia coli expec is rising globally, which is a major public health concern.
Mar 08, 2019 the bla ctxm gene was detected in klebsiella oxytoca, citrobacter freundii complex, kluyvera ascorbata and kluyvera georgiana, the latter two being the progenitor of bla ctxm genes. In fact, it has been regarded alternatively as saprophytic, opportunistic, or pathogenic. The patient, a 23 yearold male, was admitted to hospital with a temperature of 39c, abdominal pain, nausea and vomiting. Kluyvera species are described infrequently in association with clinically significant infections, and infections caused by these gram negative rods are especially rare in immunocompetent children. Natural antimicrobial susceptibility patterns of kluyvera. Get a printable copy pdf file of the complete article 2. Colicins are plasmidencoded antibiotics that are produced by and kill escherichia coli and other related species. Polymicrobial infection with kluyvera species secondary to. View enhanced pdf access article on wiley online library html view download pdf. Kluyvera 2 kluyvera ascorbata 2 kluyvera cryocrescens 2 kluyvera georgiana 2 kluyvera intermedia pdf kluyvera cryocrescens was isolated from a blood culture drawn from a patient with a broviac catheterrelated infection. Infections due to kluyvera species ascp check sample mb 104 article pdf available in american journal of clinical pathology 534. Kluyvera is a rare infection in the upper extremity. Canadian journal of microbiology nrc research press. Absolute concordance 100% was also observed in the case of isolates with phenotypes compatible with the presence of a carbapenemase with or without an esbl inferred by susceptibility patterns and phenotypic inhibitory profiles.
In addition, the gene sequences adjacent to the ctxm genes of enterobacteriaceae are also similar to those surrounding the. Pdf chromosomeencoded ctxm3 from kluyvera ascorbata. Largescale metagenomicbased study of antibiotic resistance. Kluyvera, a new redefined genus in the family enterobacteriaceae. Infections caused by kluyvera species in humans pdf. Globally, rates of esblproducing enterobacteriaceae are rising. The organism has been isolated from various clinical specimens, but its significance has not been clearly established. Escherichia fergusonii 2, kluyvera ascorbata 2, citrobacter braakii 2, cronobacter sakazakii 1, and klebsiella aerogenes 1 fig. We report the case of a 64yearold africanamerican male diagnosed with severe sepsis due to a multidrug resistant kluyvera ascorbata. If you do not see its contents the file may be temporarily unavailable at the journal website or you do not have a pdf plugin installed and enabled in your browser. List of bacteria and similar organisms presenting at the. Characterization of buttiauxella and kluyvera species by.
The esbltype bla shv38 gene was present in klebsiella pneumoniae and a bla tem variant gene exhibiting an esbl phenotype was present in e. Kluyvera is a gram negative, facultatively anaerobic bacterial and motile genus from the family of enterobacteriaceae which have peritrichous flagella. Kluyvera georgiana, kluyvera cochleae, kluyvera ascorbata and kluyvera cryocrescens, were sequenced. A search of the literature documents only a few clinical. Biomerieux and the biomerieux logo, etest, myla, villnk, and vitek are used pending andor registered trademarks belonging to biomerieux, or one of its sub. Pdf kluyvera cryocrescens was isolated from a blood culture drawn from a patient with a broviac catheterrelated infection. Found in water, soil, and sewage and in healthcare environments, most human infections are due to k. Although it causes infections infrequently, it is responsible for causing a wide range of infections including severe sepsis 1,2. Originally identified as an opportunistic pathogen, the virulence of kluyvera has been debated. Kluyvera is a relatively newly described genus in the family enterobacteriaceae that infrequently causes infections in humans.
The kit contains 16 biochemical tests, which are placed in 2 rows doublestrips of a. High levels of colicin resistance in escherichia coli. Riley department of ecology and evolutionary biology, yale university, new haven, connecticut 06511 abstract. Use of faropenem as an indicator of carbapenemase activity in.
Kluyvera ascorbata infection in a neonate fumihiro ochi. Although it causes infections infrequently, it is responsible for causing a. Broviac catheter infection with kluyvera cryocrescens. Pdf kluyvera is a relatively newly described member of the enterobacteriaceae family that rarely causes infections in humans. Antimicrobial agents active against kluyvera strains include thirdgeneration cephalosporins, fluoroquinolones, and. By deoxyribonucleic aciddeoxyribonucleic acid hybridization, strains of kluyvera were divided into three groups. In the pediatric population, it is described in association with clinically significant infections ranging from urinary tract infections to sepsis with. Nosocomial outbreak of kluyvera cryocrescens bacteremia. Mergepdf can also allows to set metadata and encrypt your merged document.
Kluyvera ascorbata is a gram negative microorganism belonging to the family enterobacteriaceae. Multistate study of enterobacteriaceae harboring extended. Kluyvera cryocrescens is a bacterium, the type species of its genus. Pdf files are for viewing not editing, if you have. We report the occurrence of kluyvera ascorbata urinary tact infection, presenting as acute pyelonephritis, in a 19monthold girl without underlying uropathy.1124 356 248 758 123 1452 1473 293 1246 670 1094 1349 1117 921 751 943 415 600 299 1075 1382 27 1149 49 893 953 1414 91